WEKO3
アイテム
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リンゴ青カビ病菌Penicillium expansum O-385-10によるペクチン分解酵素の生産とその酵素化学的性質
https://wayo.repo.nii.ac.jp/records/608
https://wayo.repo.nii.ac.jp/records/6081e4fe859-e708-454f-9416-5a3af4eb283a
名前 / ファイル | ライセンス | アクション |
---|---|---|
KJ00004421319.pdf (587.3 kB)
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2003-03-31 | |||||
タイトル | ||||||
タイトル | リンゴ青カビ病菌Penicillium expansum O-385-10によるペクチン分解酵素の生産とその酵素化学的性質 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Production of Pectin Degrading Enzymes by an Apple Blue Mold, Penicillium expansum O-385-10, and Their Properties | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | リンゴ青カビ病菌 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | リンゴ果実腐敗 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Penicillium expansum | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ペクチン分解酵素 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ポリガラクツロナーゼ | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | apple blue mold | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | apple fruit rotting | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Penicillium expansum | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | pectin degrading enzyme | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | polygalacturonase | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
著者 |
大野, 信子
× 大野, 信子× 岡留, 美穂× 李, 晶 |
|||||
著者所属(日) | ||||||
家政学部健康栄養学科 | ||||||
著者所属(日) | ||||||
家政学部健康栄養学科 | ||||||
著者所属(日) | ||||||
千葉大学園芸学部微生物工学研究室 | ||||||
抄録(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | リンゴ果実青カビ病菌Penicillium expansumの酵素の生産とペクチン分解酵素を精製し、酵素化学的性質を調べた。供試菌株は、ペクチンー無機塩類培地で、比較的短時間に、培養濾液の中に、ポリガラクツロナーゼを生産した。本菌株を窒素源としてリン酸アンモニウム(0.5%)、ペクチン(2.0%)含む無機塩類培地を用いて30℃において、4日間振とう培養した場合、培養液中の総ポリガラクツロナーゼの活性が最大(1.56U/ml)に達した。培養濾液中からDEAE-セルロースクロマトグラフィーで2つの活性画分(ポリガラクツロナーゼI、II)を精製した。それぞれポリガラクツロナーゼIとIIの活性の最適pHは4.8と5.5、最適温度は同じく40℃であった。両酵素とも0~40℃、pH3~7.5の範囲で安定であった。両酵素の活性は1mM Ca^<2+>、1mM Mg^<2+>によってそれぞれ約50~60%と約70~80%までに阻害された。 | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | The productivity of pectin degrading enzyme in an apple fruit blue mold, Penicillium expansion and properties of the partially purified enzymes were examined. In the pectin-inorganic salt medium, the organism produced extracellular polygalacturonases. The activity of total polygalacturonase in the culture solution was achieved largest (1.56 U/ml), when it was incubated in the medium containing pectin (2.0%) and ammonium phosphate (0.5%) at 30℃ for 4 days. Two active fractions (polygalacturonase I and II) were purified from the culture filtrate by DEAE-cellulose chromatography. The optimum pHs for the activities of I and II were 4.8 and 5.5, and the optimum temperatures were about 40℃. Both enzymes were stable within 0~40℃ and pH 3~7.5. Their activities were remarkably inhibited by 1 mM Ca^<2+> and 1 mM Mg^<2+>. | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN1012144X | |||||
書誌情報 |
和洋女子大学紀要. 家政系編 巻 43, p. 203-212, 発行日 2003-03-31 |